Modeling of Tau-Mediated Synaptic and Neuronal Degeneration in Alzheimer's Disease
Figure 3
Tau-mediated toxicity depends on the microtubule binding domain. Intracerebral injection of AAV-Tau.255 (10e8 tu) into wild-type mice to express a truncated form of protein Tau devoid of the microtubule binding and C-terminal domains, did not cause appreciable neurodegeneration nor inflammation. Shown are immunohistochemical stainings at 3 months p.i. for human Tau (HT7), GFAP, and MHCII as indicators of astrogliosis and microgliosis, respectively [30]. Neurons expressing truncated protein Tau still maintain neuronal nuclei (NeuN) despite changes in nuclear morphology (hematoxylin) and in content of Nissl substance (lower panels).
