Staging of Alzheimer's Pathology in Triple Transgenic Mice: A Light and Electron Microscopic Analysis

Figure 1

Photomicrographs of 6E10, AT8, PHF-1, Alz50, and AT180 intraneuronal and neuropil immunostaining in the CA1 field of the hippocampus and basolateral nucleus of the amygdala (BLA) at 3 weeks, 2–4-, and 8-9-month-old male 3xTg-AD mice. Note the differences in immunoreactivity between immersion and perfusion-fixed tissue in 3-week-old 3xTg-AD mice for 6E10 ((a)–(d)), AT8 ((e)–(h)), and PHF-1 ((i)–(l)). Alz50 ((m)–(r)) and AT180 ((s)–(x)) immunoreactivity was increased with age-dependent increases in the ventral hippocampus, ((m), (o), (q), and (s), (u), (w)) and basolateral amygdala, ((n), (p), (r) and (t), (v), (x)). Abbreviation: DG: dentate gyrus. Scale bar = 100 𝜇m.