Effects of Immunomodulatory Substances on Phagocytosis of A𝛽_{𝟏-𝟒𝟐} by Human Microglia

Figure 2

Uptake of Aβ _1–42 in human microglial cells. The confocal micrograph (63×) shows fixed CHME3 microglial cells demonstrating intracellular lysosomal location of HiLyte488-conjugated Aβ _1–42 (green filter). Lysosomes were visualized by staining with an antibody against lysosome-associated membrane protein-2 (lamp-2) and Cy3-conjugated secondary antibodies (red filter). Yellow colour thus indicates colocalization of lamp-2 and HiLyte488-conjugated Aβ _1–42.