Effects of Immunomodulatory Substances on Phagocytosis of A𝛽_{𝟏-𝟒𝟐} by Human Microglia

Figure 3

(a)-(b) Uptake of Aβ _1–42 (a) and the concurrent secretion of interleukin-6 (IL-6) and brain-derived neurotrophic factor (BDNF) (b) by human CHME3 microglial cells at 24 and 96 hours. The cells were incubated with concentrations of Aβ _1–42, ranging between 0.1 and 5 𝜇g/mL, or vehicle. The cells were harvested and the medium collected at 24 and 96 hours. In (a), the data are expressed as the proportion of cells with intracellular Aβ _1–42, 𝑛=4. In (b), the data are expressed as % of control (vehicle) set at 100% and shown as median ± percentiles (25%–75% and 10%–90%). There was a significant effect of the treatment on the uptake of Aβ _1–42 at 24 hours (𝑃=.0045) and at 96 hours (𝑃=.0375). The secretion of BDNF was also significantly altered by the treatment at 24 hours (𝑃=.0102) and at 96 hours (𝑃=.0375). Statistical difference from Aβ _1–42, 5 𝜇g/mL, is indicated by (𝑃<.01); statistical difference from vehicle is indicated by ^∗∗(𝑃<.01).

(a)

(b)