Effects of Immunomodulatory Substances on Phagocytosis of A by Human Microglia
Figure 5
(a)-(b) Effects of incubation with interleukin-1β (IL-1β) and interferon-γ (IFNγ), added 24 hours before incubation with 1 g/mL Aβ 1–42 (black boxes) or vehicle (white boxes), on secreted levels of IL-6 (a) and BDNF (b) from CHME3 microglial cells. The cells were incubated with 1 g/mL HiLyte488-conjugated Aβ 1–42 following prestimulation for 24 hours with 50 ng/mL IL-1β and 50 ng/mL IFNγ. The cells were harvested at 24, 48, 72, and 96 hours after addition of Aβ 1–42. The data are expressed as % uptake of control vehicle set at 100% and shown as median percentiles (25%–75% and 10%–90%), for 24 hours and 48 hours and for 72 and 96 hours. A statistically significant effect of treatment was found by Kruskal-Wallis ANOVA at 24 hours , at 72 hours , and at 96 hours . Statistical difference from control is indicated by ; difference from Aβ 1–42 is indicated by and . indicates statistical difference between IL-1β and IL-1β IFNγ.
