Chronic Cerebral Ischaemia Forms New Cholinergic Mechanisms of Learning and Memory

Table 1

ChAT activity and protein content in the subfractions of the synaptic membranes and the synaptoplasm of light and heavy synaptosomes of the cortex and the hippocampus in the spatial contextual (I, Contextual) and the spatial cued (II, Cued) biochemical subgroups of rats under the normal conditions. CL: the cortical light synaptosomes fraction; CH: the cortical heavy synaptosomes fraction; HL: the hippocampal light synaptosomes fraction; HH: the hippocampal heavy synaptosomes fraction; sm and Sp: the synaptic membranes and the synaptoplasm subfractions, respectively. The values of ChAT activity (nmoles acetylcholine/min) and protein content (mg) were expressed in 1 g of wet weight of tissue. In the contextual/cued biochemical subgroups 𝑛=5/4, respectively. 𝑃<.05, significant differences between the contextual and cued biochemical subgroups by the Fisher’s Exact test.

(a)

		I, Contextual		II, Cued		II/I%	𝑃
ChAT Activity		mean	SEM	mean	SEM
CL	sm	1.16	0.18	0.91	0.12	78%	>.05
	Sp	5.57	0.36	5.97	0.46	107%	>.05
CH	sm	0.054	0.008	0.048	0.004	89%	<.05
	Sp	0.367	0.037	0.352	0.021	96%	>.05
HL	sm	0.395	0.024	0.320	0.013	81%	<.05
	Sp	4.34	0.129	4.21	0.129	97%	>.05
HH	sm	0.022	0.004	0.017	0.003	77%	>.05
	Sp	0.410	0.038	0.415	0.040	101%	>.05

(b)

		I, Contextual		II, Cued		II/I%	𝑃
Protein content		mean	SEM	mean	SEM
CL	sm	5.69	0.55	5.00	0.45	88%	>.05
	Sp	4.01	0.14	4.34	0.11	108%	>.05
CH	sm	0.629	0.054	0.677	0.086	108%	>.05
	Sp	2.150	0.054	2.13	0.055	99%	>.05
HL	sm	7.10	0.304	7.49	0.304	105%	>.05
	Sp	2.90	0.195	2.95	0.139	102%	>.05
HH	sm	3.07	0.432	3.45	0.214	112%	>.05
	Sp	2.72	0.104	2.62	0.077	96%	>.05