The Pathological Roles of Ganglioside Metabolism in Alzheimer's Disease: Effects of Gangliosides on Neurogenesis
Figure 3
Effects of low (a) and high (b) concentrations of GM1 and Aβ1–40 on NECs. Basic fibroblast growth factor (bFGF; 0 or 5 ng/mL) was added as a mitogen of NECs. The number of NECs cultured in the presence of bFGF for 4 days was estimated by WST-8 assay with (a) GM1 (0, 1, 5 or 10 μM) and Aβ1–40 (0, 1 or 5 μM); with (b) GM1 (0 or 40 μM) and Aβ1–40 (0 or 10 μM). The spectrophotometric absorbance (Abs.) measured at the wavelength of 450 nm (reference, 650 nm) by this assay is highly correlated with the number of living NECs [113]. (Reproduced from [114] with permission).
