Research Article

AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5

Figure 1

(a) RNA was isolated from Neuro 2A cells transfected with either GFP or AICD-GFP. The first strand cDNA was synthesized using random hexamer primers and reverse transcriptase. Using that c-DNA as template, expression of PTCH1 and TRPC5 were also checked by real-time PCR in both the cell lines. The relative quantification of both the genes in AICD-GFP transfected cell compared to only GFP transfected cell were expressed in terms of values after normalization with respect to internal control (beta-actin gene) and plotted in log scale (log10(Fold change)) indicated and indicated . (b) Proteins were prepared from both GFP and AICD-GFP transfected cells after 24 hours of transfection, run on SDS-PAGE and western blot was done with antibody against PTCH1 (i) as well as TRPC5 (iii) and beta actin as loading control in both the cases. Fold change was calculated by densitometry analysis taking beta-actin as loading control (ii) and (iv). indicated and indicated .
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(a)
239453.fig.001b
(b)