Intracellular APP Domain Regulates Serine-Palmitoyl-CoA Transferase Expression and Is Affected in Alzheimer's Disease
Figure 2
SPT activity and expression in PS1/2- and APP/APLP2-deficient cells. (a) SPT activity in mouse embryonic fibroblasts devoid of PS1 and PS2 (MEF PS1/2−/−) and MEF PS1/2−/− retransfected with PS1 (MEF PS1r). (b) SPT activity in mouse embryonic fibroblasts lacking APP and the APP-like protein APLP2 (MEF APP/APLP2−/−) and corresponding wild-type cells (MEF WT). (c) RT-PCR analysis of SPTLC1 and SPTLC2 expression, the two subunits of SPT, in MEF PS1/2−/− and MEF PS1r. (d) SPTLC1 and SPTLC2 expression in MEF APP/APLP2−/− and MEF WT.
