Intracellular APP Domain Regulates Serine-Palmitoyl-CoA Transferase Expression and Is Affected in Alzheimer's Disease

Figure 5

SPT expression in Alzheimer’s disease. (a) Mouse embryonic fibroblasts devoid of PS1 and PS2 were retransfected with the familial PS1 mutations PS1 E280A, PS1 A285V, PS1 T354I (MEF PS-FAD), and PS1 wild-type (MEF PS1r), respectively. All PS1 mutations are known to cause early onset AD and show increased expression of SPTLC2. (b) Human postmortem PS-FAD brains, caused by the mutations I143T, L174R and L286V show increased SPTLC2 expression compared to age- and gender-matched control brains (+/− 10 years). (c) Analysis of SPTLC2 expression in 40 sporadic AD human postmortem brains compared to age- and gender-matched control brains (+/− 10 years). Pairwise normalization with the respective age- and gender-matched controls.