Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference

Figure 10

Comparison of the activity of siRNAs targeted Mut-PSEN1 gene (a) with their thermodynamic data (b). siRNAs were fully complementary to the Mut-PSEN1 transcript. Differences between the free Gibbs energy of 5′-end and 3′-end of the duplex (resp. to the polarity of the antisense strand) were calculated for three and for four consecutive base pairs: (ΔΔG₃₇^∘_C=ΔG₃₇^∘_C(5^′-end)−ΔG₃₇^∘_C(3^′-end)) (compare with Table 2).

(a)

(b)