Research Article

Ceramide and Related-Sphingolipid Levels Are Not Altered in Disease-Associated Brain Regions of APPSL and APPSL/PS1M146L Mouse Models of Alzheimer's Disease: Relationship with the Lack of Neurodegeneration?

Figure 2

Levels of NFA-ceramides and 2-HFA-ceramides (a–c) and other related-sphingolipids (SM, GalCer, and sulfatides) (d–f) in the cortex, hippocampus, and cerebellum of 12-month-old APPSL/PS1M146L, PS1M146L mice, and age-matched nontransgenic mice. WT: wild-type control mice. Each sphingolipid class was analyzed by HPTLC and their respective concentrations were calculated from standard curves after densitometric scanning of the plates as described under Section 2. Values are expressed as the means ± S.D. There was no significant difference in all sphingolipid levels between wild-type (WT), PS1M146L, and APPSL/PS1M146L mice in either cortex, hippocampus, or cerebellum (Dunn’s multiple comparison test following a Kruskall-Wallis test).
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