Ceramide and Related-Sphingolipid Levels Are Not Altered in Disease-Associated Brain Regions of APPSL and APPSL/PS1M146L Mouse Models of Alzheimer's Disease: Relationship with the Lack of Neurodegeneration?
Figure 4
Examples of HPTLC profiles of sphingolipids from different brain regions of nontransgenic and transgenic mice. (a) Sulfatides from cerebral cortex of 12-month-old APPSL/PS1M146L, PS1M146L, and their age-matched wild-type (WT) control mice. (b) Galactosylceramides (GalCer) from hippocampus of 24-month-old APPSL mice and age-matched WT control mice. Galcer and sulfatides were developed in chloroform-methanol-water (65 : 25 : 4), sprayed with orcinol-H2SO4 reagent, and then heated. Both Galcer and sulfatides* appear as two bands corresponding to the 2-HFA- (lower band) and NFA-containing galactolipids (upper band), respectively. *Standard sulfatides from bovine brain resolved in three bands, as a result of different fatty acid chain length. (c) Sphingomyelins (SM) from cerebellum of 24-month-old APPSL mice and age-matched WT control mice; SMs were developed with chloroform-methanol-water (60 : 35 : 8) (v/v/v) and visualized with sulfuric acid –CuSO4-ammonium molybdate spray reagent followed by heating. SMs appear as two bands which result of the differences in the fatty acid chain length. Std: standard sulfatides, GalCer, and SM, respectively.