Formation of Toxic Amyloid Fibrils by Amyloid β-Protein on Ganglioside Clusters
Figure 3
Comparison between Mem-fibrils and Sol-fibrils. Data taken from [20]. (a) Fluorescence spectra of ANS (5.0 μM) in PBS were measured in the absence or presence of Mem-fibrils and Sol-fibrils of Aβ-(1–40) (2.5 μM) with an excitation wavelength of 350 nm. The binding of the dye to a hydrophobic surface results in an enhancement in fluorescence intensity. (b) Aβ-(1–40) fibrils (25 μM) were incubated with NGF-differentiated PC12 cells for 30 min. Binding of Aβ-(1–40) fibrils to cells was evaluated by fluorescence intensity of Congo red per cell (mean ± S.E.; , ). (c) Aβ-(1–40) fibrils (25 μM) were incubated with NGF-differentiated PC12 cells for 24 h. Aβ cytotoxicity was estimated with fluorescence intensity of the live cell marker calcein (mean ± S.E.; ; against vehicle treatment).
