Research Article
Association between the c. 2495 A>G ATP7B Polymorphism and Sporadic Alzheimer's Disease
Table 1
Oligonucleotides sequences.
| | Primer forward for sequencing | Primer reverse for sequencing | Annealing temperature | PCR product |
| Exon 2a | 5'AGAGGCCGTCATCACTTATC 3′ | 5'CAATGGCAATCAGAGTGGTA 3' | 57°C | 255 bp | Exon 2b | 5'AGCTCCTAGGGGTTCAAAGT 3′ | 5' CAAGGAAAGTTTGCAGGATT 3’ | 57°C | 584 bp | Exon 5 | 5'TTTCACAGGCTTTCCTTGAT 3′ | 5' ATTTCCATGGGAAAAGTTGA 3' | 53°C | 336 bp | Exon 8 | 5'CGACTGTGCACAAAGCTAGA 3′ | 5'CATGGTGTTCAGAGGAAGTG 3' | 54°C | 386 bp | Exon 10 | 5’CAGCTGGCCTAGAACCTGAC 3′ | 5'TATCCTCCTGAGGGAACAT 3' | 53°C | 234 bp | Exon 14 | 5'CTGTGCAGGTGTCTTGTTTC 3′ | 5' TTTTCCAGACCACACAGAGA 3' | 57°C | 407 bp | Exon 16 | 5'TGTCCTAAAGGATGCTGTCA 3′ | 5'GGAAAACAGGCCTGAAATTA 3' | 55°C | 451 bp |
| | Primer forward for allele-specific PCR | Primer reverse for allele-specific PCR | | | Exon 10 | 5’CAGCTGGCCTAGAACCTGACCC 3’ | 5’GAAACTTTCCCCCAGGGACCACCT 3’ 5’ACTTTCCCCCAGGGACCACCC 3’ | 63°C | 141 bp | Beta actin | 5'GTCACATCCAGGGTCCTCAC 3' | 5’CACCTTCACCGTTCCAGTTT 3' | 65°C | 350 bp |
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