Accumulation of Vesicle-Associated Human Tau in Distal Dendrites Drives Degeneration and Tau Secretion in an In Situ Cellular Tauopathy Model
Figure 6
Accumulation of exogenous tau in the distal dendrites of high tau expressing ABCs is correlated with localized MT loss in distal dendrites and tau secretion. (a) left: Dendrite showing distal tau accumulation relative to tubulin level as an anti-GFP: DM1A signal ratio (Volocity), right: a graph summarizing the selective distal dendritic tau accumulation as a function of tau expression level (Bars: SEM). (b) left: A high-expressing ABC shows extensive tau secretion (asterisks) from morphologically intact dendrites near and immediately proximal to the POD. The graph to the right shows the relative intensity of tubulin (green, DM1A) versus tau (red, GFP) as a function of distance from the POD. Intact dendrites immediately proximal to the POD show a combination of recent MT loss and tau accumulation. Tubulin immunolabel levels were normalized to those in adjacent neuropil as described in the Methods section. Right: Low and high expressing ABCs were scored (+/) for degeneration and the presence of peridendritic secreted tau deposits. High tau expression (Stage 2+) was strongly correlated with secretion (bottom, asterisks , chi-square test).
