Figure 4: An increase in active caspase-3 levels induced by 2 hours of OGD was diminished by NAP treatment ( M). Cultures were treated with M NAP or with 20 μM QVD-OPH (broad spectrum caspase inhibitor) exposed to OGD insult for 2 hours. Proteins were extracted and analyzed using immunoblot with a specific antiactive caspase-3 antibody. A representative blot of active caspase-3 antibody is exhibited in (a). (ANOVA with post hoc LSD, ) (b).