Tau and Caspase 3 as Targets for Neuroprotection

Figure 5

2 hours of OGD caused an increase in p-tau²⁰² levels, prevented by either NAP or QVD-OPH treatment. Cultures were treated with 10⁻⁵ M NAP or with 20 μM QVD-OPH (broad spectrum caspase inhibitor, indicated as OGD+Q) and exposed to OGD insult for 2 hours. Proteins were extracted and analyzed using immunoblot with a specific anti-p-tau²⁰² and antitotal tau antibodies. A representative blot of p-tau²⁰² and total tau is exhibited in (a). P-tau levels were quantified and normalized to total tau levels (ANOVA with post hoc LSD, 𝑃≤0.05) (b).

(a)

(b)