About this Journal Submit a Manuscript Table of Contents
International Journal of Alzheimer’s Disease
Volume 2012 (2012), Article ID 604141, 10 pages
http://dx.doi.org/10.1155/2012/604141
Research Article

Reliable Measurements of the β-Amyloid Pool in Blood Could Help in the Early Diagnosis of AD

1Araclon Biotech Ltd., I + D Laboratory, Zaragoza, Spain
2Araclon Biotech Ltd., Proteomic Laboratory, CIBIR Logroño, Spain
3Alzheimer Research Center and Memory Clinic, Fundació ACE, Institut Català de Neurciències Aplicades, Barcelona, Spain

Received 13 December 2011; Revised 7 May 2012; Accepted 9 May 2012

Academic Editor: Seishi Terada

Copyright © 2012 Pedro Pesini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study was aimed at assessing the capability of Aβ1-40 and Aβ1-42 levels in undiluted plasma (UP), diluted plasma (DP), and cell bound (CB) to distinguish between early stages of Alzheimer's disease (AD), amnesic mild cognitive impairment (MCI), and healthy control (HC). Four blood samples from each participant were collected during one month and the levels of Aβ1-40 and Aβ1-42 were determined by a blinded proprietary ELISA sandwich (Araclon Biotech. Zaragoza, Spain). First striking result was that the amount of Aβ1-40 and Aβ1-42 in UP represented only a small proportion (~15%) of the total beta-amyloid pool in blood (βAPB) described here as the sum of Aβ1-40 and Aβ1-42 in blood where they are free in plasma, bound to plasma proteins, and bound to blood cells. Furthermore, we found that levels of Aβ1-40 and Aβ1-42 in UP, DP, and CB were significantly higher in MCI when compared to HC. On average, the total βAPB was 1.8 times higher in MCI than in HC (P=0.03) and allowed to discriminate between MCI and HC with a sensitivity and specificity over 80%. Thus, quantification of several markers of the βAPB could be useful and reliable in the discrimination between MCI and HC.