Microglial Amyloid-𝜷1-40 Phagocytosis Dysfunction Is Caused by High-Mobility Group Box Protein-1: Implications for the Pathological Progression of Alzheimer’s Disease

Figure 1

Binding affinity of HMGB1 for A𝛽40. (a, b) After incubation of HMGB1, A𝛽40, and the mixture of HMGB1 with A𝛽40 for 0, 6, and 24 h, samples were analyzed by Western blot analysis using the anti-HMGB1 antibody (a) or anti-A𝛽 antibody (b). (c, d) After incubation of HMGB1, A𝛽40, and the mixture of HMGB with A𝛽40 for 24 h, samples were immunoprecipitated using the anti-HMGB1 antibody (c) or anti-A𝛽 antibody (d). The precipitates were then analyzed by Western blot analysis using the anti-HMGB1 antibody and anti-A𝛽 antibody.