Figure 1: Binding affinity of HMGB1 for A40. (a, b) After incubation of HMGB1, A40, and the mixture of HMGB1 with A40 for 0, 6, and 24 h, samples were analyzed by Western blot analysis using the anti-HMGB1 antibody (a) or anti-A antibody (b). (c, d) After incubation of HMGB1, A40, and the mixture of HMGB with A40 for 24 h, samples were immunoprecipitated using the anti-HMGB1 antibody (c) or anti-A antibody (d). The precipitates were then analyzed by Western blot analysis using the anti-HMGB1 antibody and anti-A antibody.