685739.fig.001
Figure 1: Binding affinity of HMGB1 for A 40. (a, b) After incubation of HMGB1, A 40, and the mixture of HMGB1 with A 40 for 0, 6, and 24 h, samples were analyzed by Western blot analysis using the anti-HMGB1 antibody (a) or anti-A antibody (b). (c, d) After incubation of HMGB1, A 40, and the mixture of HMGB with A 40 for 24 h, samples were immunoprecipitated using the anti-HMGB1 antibody (c) or anti-A antibody (d). The precipitates were then analyzed by Western blot analysis using the anti-HMGB1 antibody and anti-A antibody.