(a)

(b)

Figure 3: Effect of exogenous HMGB1 on microglial A phagocytosis analyzed by Western blot. Rat microglia were incubated with the vehicle, HMGB1, A40, or A40 and HMGB1 for 12 h. Microglial cell lysate (a) and conditioned medium (b) were then subjected to Western blot analysis using the anti-A antibody, and then the amounts of A40 inside (a) and outside microglia (b) were semiquantitatively measured. *, *** versus treatment with A40 alone.