685739.fig.004a
(a)
685739.fig.004b
(b)
Figure 4: A degradation with the microglial cytosolic fraction. (a) A 40 and A 42 were incubated with the rat microglial cytosolic fraction for 0, 6, and 24 h. After incubation, samples were subjected to Western blot analysis using the anti-A antibody, and the amount of A was semiquantitatively measured. ** , *** versus time point 0 h. ††† versus A 40 at time point 24 h. (b) A 40 and A 40 with HMGB1 were incubated with rat microglial cytosolic fractions for 0, 6, and 24 h. After incubation, the samples were subjected to Western blot analysis using the anti-A antibody, and the amount of A was semiquantitatively measured. ** , *** versus A 40 alone at each time point.