Caspase-Mediated Truncation of Tau Potentiates Aggregation
Figure 2
Tau truncation by caspases. (a) depicts GFP-tagged 3 PO tau. The caspase cleavage sites reported in AD brains and the epitopes recognized by the antibodies used for the truncation detection in this study are noted. GFP tagged 3PO tau is depicted. Caspase-3 has been reported to cleave tau at D25 and D241. Caspase-6 has been reported to cleave tau at D13, D402, or D421. (b) presents representative immunoblots of lysates from cell expressing GFP-3PO with and without treatment with staurosporine or Z-VAD-fmk as indicated. Migration of molecular weight standards is indicated on the left of each blot. The accompanying graphs presents the mean intensities (±SEM) derived from 4 independent immunoblots. The relative intensities of the uppermost bands from anti-GFP, C3, and Tau46 were normalized to those of Tau5; lower-molecular weight breakdown products were not considered in these densitometric analyses. An asterisk indicates statistical significance (, Student’s t test).
