The Complexity of Sporadic Alzheimer’s Disease Pathogenesis: The Role of RAGE as Therapeutic Target to Promote Neuroprotection by Inhibiting Neurovascular Dysfunction

Figure 1

TXNIP is overexpressed in the hippocampus of the 5xFAD mice. Top: Floating brain slices were incubated 24 h with mouse anti-TXNIP monoclonal antibody in PBS 3% BSA, 0.1% Triton X-100 (blocking) at 4°C. Slides were washed 3 times for 15 min with PBS and incubated for 45 min with TRITC-conjugated secondary antibody (red). Nuclei were stained by incubating the slides with Hoecst (blue) together with the secondary antibody. Slides were mounted using mounting medium and analyzed with confocal microscopy (Zeiss). Center: Confocal analysis and 3 dimensional reconstruction (Zen software of Zeiss) of TXNIP staining in the hippocampus. Bottom: Floating brain slices were incubated 2 h at room temperature with rabbit anti-GFAP polyclonal antibody in PBS 3% BSA, 0.1% Triton X-100 (blocking). Slides were washed 3 times for 15 min with PBS and incubated for 45 min with FITC-conjugated secondary antibody (green). Slides were mounted using mounting medium and analyzed with confocal microscopy (Zeiss). These results are representative of 4 independent experiments (4 animals).