Silencing of Amyloid Precursor Protein Expression Using a New Engineered Delta Ribozyme

Figure 3

Expression of APP-SOFA-HDV ribozymes in the HEK cell line. Primer extension analysis of total cellular RNA from cells transfected with four selected APP-SOFA-HDV ribozymes from the ribozyme collection (APP-SOFA-HDV-Rz276, APP-SOFA-HDV-Rz753, APP-SOFA-HDV-Rz756, and APP-SOFA-HDV-Rz885). Transcripts corresponding to HDV-ribozymes were detected with HDV-Rz primers (5′-GGGTCCCTTAGCCATGCGCGAACG-3′). U6 primer (5′-GGCCATGCTAATCTTCTCTG-3′) was also used as a positive control, which yielded signals corresponding to endogenous U6 snRNA. Note that all of the 4 selected SOFA-HDV ribozymes were expressed (lanes 1 to 4). A pRNAT empty vector, was used as a negative control.