Silencing of Amyloid Precursor Protein Expression Using a New Engineered Delta Ribozyme
Figure 5
Rz-APP-X ribozymes expression reduces APP levels. (a) Western blotting analysis performed using equal amounts of 20 g of extracted protein. APP was immunodetected using a polyclonal antibody (Sigma) recognizing the C-terminus of human APP. The tubulin (-tubulin) controlled the amount of sample loaded in each lane. The highest APP reduction (approximately 85%) was obtained with Rz-APP-276 and Rz-APP-753. (b) Densitometric quantification of the APP lanes in the blot from (a). Relative density unit values were obtained by standardization with the corresponding -tubulin protein band in each lane. The results in (b) are presented as the means ± SEM from 3 independent experiments. **.
