Alpha 1-Antichymotrypsin, an Inflammatory Protein Overexpressed in the Brains of Patients with Alzheimer’s Disease, Induces Tau Hyperphosphorylation through c-Jun N-Terminal Kinase Activation
Figure 4
ACT-mediated tau phosphorylation at p-Ser202, p-Thr231, PHF-1, and p-Ser262 in cortical neurons is inhibited by JNK inhibitor: mouse cortical neurons were cultured in 8-chamber slides and preincubated with JNK Inhibitor (10 μM, 1 hour). The cells were then treated with or without 1 mg/mL ACT for 8 hours and probed with (a) p-Ser202 tau monoclonal and p-Thr231 tau polyclonal, (b) PHF-1 tau monoclonal and P-Ser262 tau polyclonal antibodies. Anti-mouse Alexa fluor 488 (green) and anti-rabbit Alexa Flour 594 (red) were used as secondary antibodies, and Hoechst was used to visualize the nuclei. Staining was visualized under a Zeiss fluorescent microscope and analyzed using AxioVision Rel. 4.8 software.
