Research Article
Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
Table 2
Pairs of primers used for PCRs.
| | Amplified gene | Primer names | 5′→3′ sequences | Annealing temperature (°C) | Product length (bp) | GenBank accession number |
| | Dog NEP (exons 4 to 9) | NEP1U20
NEP625L20 | F: ATGGGCAGATCAGAAAGTCA
R: TATCATCCGTGCCAACAAAA | 57 | 625 | JF451103 |
| | Dog IDE (exons 15 to 19) | IDE1815U20
IDE2307L20 | F: CCTCAAAGACTCACTCAACG
R: CAGCTGACTTGGAAGGAGAG | 53 | 493 | XM534963 |
| | Dog Ubi | Ubi145U20
Ubi343L20 | F: CAGCTAGAAGATGGCCGAAC
R: ACTTCTTCTTGCGGCAGTTG | 53 | 199 | AB032025 |
| | Dog NEP (for qPCR) | NEP1575U20
NEP1728L20 | F: CCGAGAAAAAGTGGACAAGG
R: ACCCCCGTAGTTCAAGGAGT | 60 | 154 | JF451103
JF713705 |
| | Dog Ubi (for qPCR) | Ubi190U20
Ubi344L20 | F: GAGTCCACCTTGCACTTGGT
R: CACTTCTTCTTGCGGCAGTT | 60 | 155 | AB032025 |
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