Nanoscale Extracellular Vesicle Analysis in Alzheimer’s Disease Diagnosis and Therapy

Figure 4

Flow cytometry histograms showing ability to specifically enrich nsEVs from plasma using streptavidin-coated Dynabeads loaded with biotinylated Abs. After plasma incubation, magnetic particles were labeled with phycoerythrin- (PE-) conjugated anti-CD9 Ab. The -axis denotes number of magnetic particle events counted by flow cytometer. The -axis denotes phycoerythrin (PE) fluorescence. Legends under histograms denote biotinylated Abs loaded onto magnetic particles. (a) corresponds to analysis of magnetic particles incubated with PBS/2.5% BSA rather than plasma; these histograms show that PE-conjugated anti-CD9 Ab has little or no propensity to nonspecifically bind Ab-loaded magnetic particles.

(a)

(b)