Expression, Purification, and Functional Characterization of Atypical Xenocin, Its Immunity Protein, and Their Domains from Xenorhabdus nematophila
Figure 4
(a) ATPase assay with immunity domain (10 kDa), hemolysin domain (32 kDa), and full length immunity protein (42 kDa). (a) Lane 1: BSA (500 ng, negative control); lane 2: Gro EL (500 ng, positive control). Lane 3: immunity domain (100 ng); lane 4: (250 ng); and lane 5, (500 ng): Lane 6: hemolysin domain (100 ng); lane 7 (250 ng) and lane 8: (500 ng). Lane 9: immunity protein (100 ng); lane 10 (250 ng) and lane 11: (500 ng). (b) Histogram prepared by IMAGE J software on the basis of band intensity.
