Lymphangiogenesis and Axillary Lymph Node Metastases Correlated with VEGF-C Expression in Two Immunocompetent Mouse Mammary Carcinoma Models
Figure 1
TEM micrographs (a and b), immunofluorecent staining (c and d), Western blot analysis (e), and RT-PCR analysis (f) of VEGF-C in the two-mouse mammary carcinoma cell lines. Both BJMC338 cells (a) and BJMC3879 cells (b) have the similar ultrastructure. BJMC338 cells (c) have minimally any activity of VEGF-C, whereas BJMC3879 cells (d) have moderate expression. Western blot analysis (e) demonstrates the same activity of VEGF-C as in (c and d). VEGF-C mRNA expression in BJMC3879 cells is higher than that of BJMC338 cells (f). Green fluorescence (FITC) indicates activity of VEGF-C, and red fluorescence (PI) shows nuclei of cells in (c and d).
