Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines
Figure 5
(a) and (b) Effect of EJAE and RRE on the expression of nuclear receptors and cell-death markers in ICI-resistant (LCC9) cell lines. Biomarker analysis results after 3 d treatment of EJAE with and without drug treatments. Data represented as fold change over the vehicle. All treatments were normalized to vehicle treatment (first lane). Vehicle treatment contained 0.01% EtOH and 0.1% DMSO. Concentrations of treatments were as follows: TAM (4-0HT) = 100 nM; ICI = 1 nM; EJAE = 100 μg/mL; RRE = 10 μg/mL; and DEL = 1 μM. Concentration of extract used showed the most significant reduction in cell proliferation of LCC9 cells after 6 d treatment. Each extract or polyphenol used for treatment was diluted 1000-fold from stock concentration. *designates a significance of compared to vehicle control.
