Preclinical Evaluation of Oncolytic Δγ134.5 Herpes Simplex Virus Expressing Interleukin-12 for Therapy of Breast Cancer Brain Metastases
Figure 2
A panel of cell lines including permissive Vero cells and 5 human breast cancer cell lines, MDA-MB-361 (M361), BT-474, SK-BR-3 (SKBR), MDA-MB-231 (M231), and ZR-75-1 (ZR75) were infected with M002 at an MOI of 5 PFU/cell. (a) Bright-field photomicrographs (100x magnification) of viral cytopathic effect mediated by M002 in the infected cells shown in comparison with healthy uninfected cells. The cytopathic effect mediated by HSV-1 (F) and R3659 (not shown) were identical to M002. (b) Replication of wild-type HSV-1 (F), IL-12-expressing HSV M002, or the non-cytokine-expressing control virus R3659 in a panel of human breast cancer cells at 48 h post-infection. Shown are the averages and standard deviations of duplicate determinations, from a representative of three experiments.
