Preclinical Evaluation of Oncolytic Δγ134.5 Herpes Simplex Virus Expressing Interleukin-12 for Therapy of Breast Cancer Brain Metastases
Figure 5
Development of an immunocompetent intracranial model of breast cancer brain metastases. Female A/J mice in two cohorts (, each) were given intracranial injections of 1 × 104 or 5 × 104 cells/5 μL. By days six (5 × 104 cohort) and nine (1 × 104) post-injection, all mice in each cohort had been euthanized due to the onset of neurological symptoms. Brains were harvested for histological examination. Shown are hematoxylin and eosin-stained sections from a representative specimen. Note the massive tumor at the injection site in the first panel (40x magnification) with associated hemorrhaging. Insets (400x) show the relatively well circumscribed border of the main tumor (a) and the more invasive character of a distant tumor nest (b).
