Table 1: Comparison between Dr. Vainer et al.’s study [8] and Ancona experience [3].

FeaturesDr. Vainer et al.’s studyAncona experience

Prostate weight and size (and volume)Yes
(not mentioned)
Yes
(yes)
Fixation enhancementSeparating the two lobesFormalin injection
Inking of the surfaceTwo colours, anterior, and posterior halvesOne colour; orientation with a cut on the right
Presectioning fixation (time)Acid formic (20 h) and 4 buffered formalin (24 h)4 buffered formalin (24 h)
Sectioning interval Approximately 3 mm (Apex and base: 5–10  mm)3 mm (Apex and base: 3 mm)
Subdivision of the slices of the prostate bodyYes, to fit standard cassettesNo (whole mounts)
Seminal vesiclesAs a minimum the apex and a cross-sectionSandwich method (all included)
Postsectioning fixation (time)4 buffered formalin (not mentioned)4 buffered formalin (24 h)
No. of cassettes/total slides (% examined)18–76 (70 )9–14 (100 )
ProcessingNot mentionedAs for regular size cassettes
Slide size (section thickness)7.5 cm by 2.5 cm (4  m)7.5 cm by 5.0 cm (5  m)
Slide staining procedureNot mentionedManual