Phenotypic and Molecular Characterization of MCF10DCIS and SUM Breast Cancer Cell Lines

Table 4

Mutational analysis Rb pathway genes for Asterand SUM cell lines.

Cell line	RB1 protein expression	Cyclin D1 transcript expression	Cyclin D1 protein expression	p16 gene; sequence	p16 transcript; expression	p16; protein; expression
SUM102PT	nd	++	nd	Deleted	+	nd
SUM1315MO2	+	+	±	Deleted	−	−
SUM149PT	+	+	±	Deleted	−	−
SUM159PT	+ +	+	+	Wild type	−	±
SUM229PE	+	+	+	Deleted	−	−
SUM185PE	+ +	+	+	Wild type	±	±
SUM190PT	nd	nd	nd	nd	nd	nd
SUM225CWN	nd	nd	nd	nd	nd	nd
SUM44PE	+	++	++	Methylated	±	±
SUM52PE	+	+	+	Mutant	+	+

Source: [48]; nd: not determined, +: normal expression, ±: barely detectable, −: no detectable expression, ++: over expression.