Review Article
Phenotypic and Molecular Characterization of MCF10DCIS and SUM Breast Cancer Cell Lines
Table 4
Mutational analysis Rb pathway genes for Asterand SUM cell lines.
| Cell line | RB1 protein expression | Cyclin D1 transcript expression | Cyclin D1 protein expression | p16 gene sequence | p16 transcript expression | p16 protein expression |
| SUM102PT | nd | ++ | nd | Deleted | + | nd | SUM1315MO2 | + | + |
± | Deleted | − | − | SUM149PT | + | + |
± | Deleted | − | − | SUM159PT | + + | + | + | Wild type | − |
± | SUM229PE | + | + | + | Deleted | − | − | SUM185PE | + + | + | + | Wild type |
± |
± | SUM190PT | nd |
nd | nd | nd | nd | nd | SUM225CWN | nd | nd | nd | nd | nd | nd | SUM44PE | + | ++ | ++ | Methylated |
± |
± | SUM52PE | + | + | + | Mutant | + | + |
|
|
Source: [48]; nd: not determined, +: normal expression, ±: barely detectable, −: no detectable expression, ++: over expression.
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