Research Article

Promoter Methylation Status of Breast Cancer Susceptibility Gene 1 and 17 Beta Hydroxysteroid Dehydrogenase Type 1 Gene in Sporadic Breast Cancer Patients

Figure 1

A representative gel picture of 2% agarose gel showing BRCA1 methylation status of two cases. Lanes 1, 2, 3, 4, 5, and 7: the presence of (500 bp) band indicates methylation of BRCA1 promoter in these specimens. Lanes 6 and 8: absence of (500 bp) band indicates the absence of methylation of BRCA1 promoter in these specimens. Lane 9: negative control. Lane 10: 100 bp DNA ladder. Lanes 1 and 5: undigested DNA of normal breast tissue specimens; lanes 2 and 6: digested DNA of normal breast tissue specimens; lanes 3 and 7: undigested DNA of cancerous breast tissue specimens; and lanes 4 and 8: digested DNA of cancerous breast tissue specimens.