Research Article

Promoter Methylation Status of Breast Cancer Susceptibility Gene 1 and 17 Beta Hydroxysteroid Dehydrogenase Type 1 Gene in Sporadic Breast Cancer Patients

Figure 2

A representative gel picture of ethidium bromide-stained 2% agarose gel showing 17βHSD-1 methylation status of two cases. Lane 1: a 100 bp DNA ladder. Lanes 2, 3, 4, 5, 6, 7, 8, and 9: the presence of (238 bp) band indicates methylation of 17βHSD-1 promoter in these specimens. Lane 10: negative control. Lanes 2 and 6: undigested DNA of normal breast tissue specimens; lanes 3 and 7: digested DNA of normal breast tissue specimens; lanes 4 and 8: undigested DNA of cancerous breast tissue specimens; and lanes 5 and 9: digested DNA of cancerous breast tissue specimens.