Genetic Diversity Assessment and Identification of New Sour Cherry Genotypes Using Intersimple Sequence Repeat Markers

Table 2

23 ISSR primers used in this study and their results.

Number	Primers	Sequence (5′-3′)	TA^1 (°C)	Total number of alleles (𝑎)	Number of polymorphic alleles (𝑏)	% Polymorphism; (𝑏/𝑎) × 100	PIC^2	Average gene diversity (Hi)	Band size range (bp)
1	ISSR-1	GTGGTGGTGGC	30	16	16	100	0.90	0.45	600–1500
2	ISSR-2	GAGAGAGAGAGAGAGAT	48	21	21	100	0.94	0.40	400–1500
3	ISSR-3	CTCTCTCTCTCTCTCTG	47	25	24	96.00	0.94	0.43	700–2500
4	ISSR-4	GAGAGAGAGAGAGAGATG	52	26	26	100	0.94	0.37	400–1400
5	ISSR-5	AGAGAGAGAGAGAGAGTT	52	21	21	100	0.94	0.36	400–2100
6	ISSR-6	CTCTCTCTCTCTG	39	29	29	100	0.94	0.40	400–2200
7	ISSR-7	CTCTCTCTCTCTCTCTTG	47	18	17	94.44	0.93	0.37	700–1700
8	ISSR-8	CACACACACACAAC	39	25	24	96.00	0.95	0.40	700–2300
9	ISSR-9	CACACACACACAGT	39	18	18	100	0.89	0.39	700–2800
10	ISSR-10	CACACACACACAGG	36	21	21	100	0.94	0.37	400–1700
11	ISSR-11	CACACACACACAAG	30	23	23	100	0.94	0.43	700–3000
12	ISSR-12	CACACACACACACACAGG	47	15	14	93.33	0.88	0.43	500–2100
13	ISSR-13	CTCTCTCTCTCTCTCTRG	53	29	29	100	0.96	0.34	400–2800
14	ISSR-14	DBDACACACACACACAC	55	29	29	100	0.95	0.42	400–2800
15	ISSR-15	HVHTCCTCCTCCTCCTCCTCCTCC	57	9	7	77.77	0.85	0.45	600–1800
16	ISSR-16	GAGAGAGAGAGAGAGAC	53	25	25	100	0.95	0.45	400–3200
17	ISSR-17	ACACACACACACACACC	53	17	17	100	0.90	0.44	600–2200
18	ISSR-18	GAGAGAGAGAGAGAGAYC	47	15	14	93.33	0.90	0.45	600–1800
19	ISSR-19	AGAGAGAGAGAGAGAGYT	52	29	29	100	0.95	0.41	280–1600
20	ISSR-20	ACACACACACACACACYG	53	16	16	100	0.90	0.43	400–2800
21	ISSR-21	CACACACACACACACART	48	24	24	100	0.94	0.39	600–1700
22	ISSR-22	GAGAGAGAGAGAGAGAYG	47	20	20	100	0.93	0.40	400–2200
23	ISSR-23	GAGAGAGAGAGAGAGACG	55	18	18	100	0.94	0.42	400–3200
	Mean	—	—	21.26	20.95	98.45	0.93	0.41	—
	Total	—	—	489	482	—	—	—	—

Note: D = (G, A, T), B = (G, T, C), H = (A, T, C), V = (G, A, C), R = (A, G), Y = (C, T), and N = (A, T, G, C).
¹Annealing temperature. ² Polymorphism Information Content.