Multiscale Characterization of the PEPCK- Mouse through 3D Cryoimaging
Figure 2
Comparison of a PEPCK- mouse and a control mouse. A coronal 2D section (no. 390 of 586) of the transgenic PEPCK- is shown in (a) while (b) shows similar coronal 2D section (no. 240 of 616) of the control mouse. Both images were composited by tiling 24 images each with an in-plane pixel size of 15.6 m. In both cases, the embedding compound OCT has been replaced with a background of black through automated image processing. Major organs like the heart (H), lungs (G), liver (L), salivary glands (R), stomach (M), spleen (P), pancreas (C), small intestine (I), colon (N), and lymph node (Y) are visible. Compared to the older PEPCK- mouse, the control mouse has large amounts of visceral (V) and subcutaneous (S) adipose tissue. The peritoneum (*) of the control mouse is thus distinctly visible (Bar = 10 mm).
