Multiscale Characterization of the PEPCK-C^mus Mouse through 3D Cryoimaging

Figure 5

Bone Morphometry. Digital resections along the femur ((a), (b)—marked *) and tibia ((c), (d)—marked +) for the PEPCK-C^mus (a, c) and control mouse (b, d) obtained through multiplanar reformatting of the 3D data. Linear distance was computed to ascertain bone lengths. The femur measured 11.46 mm (PEPCK-C^mus) and 12.41 mm (control) while the tibia measured 17.26 mm (PEPCK-C^mus) and 15.12 mm (control). The tibia to femur length ratio was 24% higher for the PEPCK-C^mus mouse as compared to the control animal. Cortical areas were measured mid diaphyseally in cross-sections at positions indicated by arrows (Bar = 2 mm).

(a)

(b)

(c)

(d)