Image Processing Techniques for Assessing Contractility in Isolated Neonatal Cardiac Myocytes

Algorithm 1

𝐯⇐imageacquisition           % using inverted phase contrast microscope

𝑁⇐frames(𝐯)                   % number of frames in video

𝑓⇐𝑣(𝐱,1)                       % read first frame

𝑚⇐mask                      % construct binary mask

𝑣(𝐱,1)=𝑚𝑣(𝐱,1)% apply binary mask to first frame

𝑉(𝐱,1)=PFFT(𝑣(𝐱,1))% apply polar FFT to first frame

𝐶(1)⇐1% correlation coefficient of first frame

𝐟𝐨𝐫𝑖=2to𝑁  do

 𝑣(𝐱,𝑖)=𝑚𝑣(𝐱,𝑖)              % apply binary mask to each frame

 𝑉(𝐱,𝑖)=PFFT(𝑣(𝐱,𝑖))             % apply polar FFT to each frame

 𝐶(𝑖)=CORR(𝑉(𝐱,1),𝑉(𝐱,𝑖))         % correlation coefficient of each frame

end for