Research Article
Estimating Cell Count and Distribution in Labeled Histological Samples Using Incremental Cell Search
Table 3
Count results of ICS and ITCN at different resolutions.
| Resolutions | Manual counts | ITCN | ICS | Cell diameter DAB (pixels) | Cell diameter DAPI (pixels) | Corresponding optical magnification | Brightfield (DAB stained) | UV (DAPI stained) | Brightfield (DAB stained) | UV (DAPI stained) | Brightfield (DAB stained) | UV (DAPI-stained) | Threshold | Average count | Threshold | Average count | Average count | average count |
| 45 | 32.4 | 400x | N/A | N/A | 0.1 | 33.2 | 0 | 306.6 | 26.5 | 376.4 | 32 | 23 | 200x | 26.0 | 411.5 | 0.2 | 33.6 | 0.1 | 261.0 | 26.2 | 375.3 | 25 | 16.2 | 100x | N/A | N/A | 0.3 | 38.0 | 0.1 | 269.0 | 26.2 | 374.6 | 16 | 11.5 | 50x | N/A | N/A | 0.8 | 36.7 | 0.1 | 288.7 | 27.7 | 376.6 | 8 | 5.75 | 12.5x | N/A | N/A | 2 | 50.0 | 0.4 | 316.0 | 27.7 | 367.0 |
| Average across resolutions: | 26.0 | 411.5 | | 38.3 | | 288.3 | 26.8 | 374.0 |
| Coefficient of variation across resolutions: | 14.2% | 16.3% | | 19.3% | | 10.9% | 8.9% | 2.7% |
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