- About this Journal ·
- Abstracting and Indexing ·
- Advance Access ·
- Aims and Scope ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents

International Journal of Biomedical Imaging

Volume 2013 (2013), Article ID 593183, 6 pages

http://dx.doi.org/10.1155/2013/593183

## Closed Contour Specular Reflection Segmentation in Laparoscopic Images

Hamburg University of Technology, Schlossstraße 20, 21079 Hamburg, Germany

Received 9 April 2013; Revised 12 July 2013; Accepted 12 July 2013

Academic Editor: Tiange Zhuang

Copyright © 2013 Jan Marek Marcinczak and Rolf-Rainer Grigat. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

#### Abstract

Segmentation of specular reflections is an essential step in endoscopic image analysis; it affects all further processing steps including segmentation, classification, and registration tasks. The dichromatic reflectance model, which is often used for specular reflection modeling, is made for dielectric materials and not for human tissue. Hence, most recent segmentation approaches rely on thresholding techniques. In this work, we first demonstrate the limited accuracy that can be achieved by thresholding techniques and propose a hybrid method which is based on closed contours and thresholding. The method has been evaluated on 269 specular reflections in 49 images which were taken from 27 real laparoscopic interventions. Our method improves the average sensitivity by 16% compared to the state-of-the-art thresholding methods.

#### 1. Introduction

One major concern in laparoscopic image processing is specular reflections which are present in the majority of laparoscopic interventions and affect all following processing. Specular reflections are most pronounced if the surface normal bisects the angle between the incident light and the camera. They are caused by moist tissue and appear as white glare or light-colored glare in the images. Many different approaches to segment specular reflections have been proposed in the previous decades. Most of them are based on the dichromatic reflection model [1, 2]. Let be the incident angle, the exitance angle, the phase angle, and the wavelength. The reflectance and model the surface reflection and the body reflection. The radiance reflected by a surface can be defined as

The dichromatic reflection model holds for dielectric surfaces and separates the spectral reflection from the geometric reflection [3–5]: where and are geometric scaling factors and and are spectral power distributions. The body reflection (diffuse reflection) and the specular reflection form linear clusters in a color histogram [6]; fitting linear subspaces to these clusters can be used to detect specular reflections in images, and the diffuse color can be reconstructed by projection. However, in practice, surface roughness and the imaging geometry make the fitting of subspaces inaccurate [7]. Additionally, the assumption of dielectric surfaces is not fulfilled by human tissue. Nevertheless, several algorithms have been proposed that use the dichromatic model in an endoscopic environment [8, 9]. However, Vogt et al. show that simple S channel thresholding in the HSV color space achieves similar accuracy on endoscopic images [10]. Several adaptive thresholding techniques have been proposed that make use of nonlinear color transformations to separate the specular reflections from bright tissue in color space [9, 11]. Most of the specular reflection segmentation algorithms have in common that thresholding is used to segment the central part of the reflections, and the bright region surrounding the reflection is segmented in a second step. In the following, we will refer to this region where specular reflection is still strong, but body reflection increases as specular lobe. Commonly, this region is segmented by applying morphological operations to the thresholded image or using region growing [12]. As mentioned by several authors, single threshold techniques have limited accuracy [13, 14]. Bright parts of the tissue commonly intersect with weak specular reflections in color space. The approach of Oh et al. [14] is similar to our approach as they distinguish between weak and strong reflections using multiple thresholds. In our approach, specular reflections are classified as weak, intermediate, and strong reflections. We demonstrate that closed contours can be used to detect weak reflections which would be missed by thresholding techniques. The segmentation of weak, intermediate, and strong reflections is combined to obtain the final segmentation.

In here, Sections 2 and 3 discuss methods and results. First, thresholding techniques for specular reflection segmentation are detailed in Sections 2.1 and 2.2. These methods are applied in conjunction with the specular lobe segmentation described in Section 2.3. Section 2.4 defines our hybrid approach combining thresholding techniques with closed contour segmentation. The results of our approach are compared to the classical techniques in Section 3.

#### 2. Methods

The most common techniques for specular reflection segmentation in endoscopic images are thresholding methods [9–11, 13, 14]. In the following sections, we detail the specular peak thresholding algorithm and outline a second thresholding method which we refer to as cone thresholding. In the evaluation, we compare these thresholding techniques to our hybrid approach which is explained in Section 2.4.

##### 2.1. Specular Peak Thresholding

A common assumption for specular reflections is that they are located in the brightest peak in the histogram of an image. Stehle selects the brightest peak of the luminance channel of the YUV color space [15], while Saint-Pierre et al. perform a nonlinear transformation. Let be an RGB image; the transformed image is defined by where is the saturation channel of using the HSV color space [16]. This transformation decreases the color values depending on their distance to the gray-axis. The transformation is based on the assumption that most specular reflections will be located close to the gray-axis and have a low saturation. This transformation increases the gap between specular reflections and tissue in the histogram [11]. The threshold is selected by the following criterion. Let be the histogram of the Y channel of and . The threshold is given by with

In [11], the threshold is directly used to detect specular reflections. However, in our experiments this segmentation was not robust; if no specular reflections are present in the image, the brightest parts of the tissue will be classified as specular reflections. Furthermore, different specular reflections might appear at different intensity levels and lead to several peaks in the high intensity range of the histogram . Therefore, we apply the two following steps to increase the robustness of the algorithm. First, we allow only thresholds . Second, we convolve with a Gaussian kernel to merge the peaks of that are caused by different specular reflections. The set of specular reflections is given by , where is the Y channel of . This approach sets the threshold according to the brightest specular peak in .

##### 2.2. Cone Thresholding

One drawback of the specular peak thresholding technique is the assumption that specular reflections are represented by a single peak in the histogram of an image. In practice, this is not always the case. Specular reflections appear at different intensity levels and can lead to several peaks in the histogram. Therefore, another technique which relies on defining a cone in the RGB color space as specular reflections can be used. The cone is located on the axis for which , which implicitly assumes a perfect white balance. Let with be an RGB image. The projection of on is given by

The set of specular reflections is then defined by

The parameters and define the tip and the slope of the cone. This detection algorithm relies on the assumption that the specular reflections are close to the gray-axis of the RGB space. The advantage of this segmentation algorithm is that it does not suffer from multiple peaks in the histogram of the image. However, one should keep in mind that the RGB color space is a hardware-dependent color space. Therefore, the parameters and need to be adjusted for different hardware.

##### 2.3. Specular Lobe Segmentation

The thresholding techniques outlined in the previous sections segment the central part of the specular reflections. To obtain a segmentation of the entire specular reflection, specular lobe segmentation needs to be applied. We use a technique similar to [11] which is based on region growing [12]. Instead of using a single threshold for the region growing, we compute thresholds based on the specular reflection intensities. For every connected component , with , where is the number of connected components in , the mean value of the connected component is estimated by

The region growing algorithm adds a pixel to the set if where is a scaling factor and is the luminance channel of the YUV color image. The final segmentation is given by . The optimal parameter for different thresholding algorithms is given in Figure 3. In the following evaluation, specular lobe segmentation is used together with the outlined thresholding algorithms.

##### 2.4. Hybrid Closed Contour Thresholding

Single threshold segmentation techniques have an upper limit of accuracy which is often caused by bright tissue which is classified as specular reflection. An increase in precision can be achieved by using different models for specular reflections. In laparoscopic videos of the liver surface, different types of specular reflections appear. The first types of reflections are large specular reflections which occur in situations where the endoscope is located very close to the organ surface (Type 1). As the image intensities at specular reflections of Type 1 are very high and often clipped in the center part, thresholding can be used to segment this type of specular reflections. Another property of this type of reflection is the slowly, radial decreasing intensity. More difficult to detect are small, weak reflections located further apart from the endoscope. These reflections are caused by moist curved organ surfaces. The intensities of these small specular reflections can be low, depending on the surface geometry and the underlying tissue. However, most of this type of reflections have a step-shaped border, which can be used for detection and precise segmentation. In the proposed segmentation algorithm, this type of specular reflections is split up into small reflections with high intensity (Type 2) and specular reflections that have low intensities even in the center (Type 3). All three types are illustrated in Figure 1. For Types 2 and 3, the contour is used to determine the segmentation boundary. For every connected component in the binary image that was created by cone thresholding, it is checked if the component is enclosed by a contour; this contour is used for segmentation. If no closed contour is found, the reflection is classified as Type 1. To detect reflections of Type 3, closed contours are used as seed points. An overview of this approach is given in Figure 2. In the following, denotes the set of pixels segmented as specular reflections by thresholding. The Canny edge detector is used to compute a binary edge map of the laparoscopic image [17]. The liver surface is smooth and lacks edges or corners. Therefore, most of the strong filter responses are caused by the boundaries of specular reflections. A morphological closing operation is used to close small gaps in the contours. Let denote the set of pixels of the connected component that is enclosed by the contours of . The set of Type 2 reflections is obtained by the connected components that contain at least one pixel of . The weak Type 3 reflections are segmented using constraints on the connected components that are not elements of . The specular reflections are given by the connected components that fulfill the following constraints:

The perimeter of is denoted by , and denotes the expectation value. The last two constraints assure that only closed contours of a specific size are considered to be specular reflections of Type 3. The first two constraints are based on the aspect that the average intensity of specular reflections is limited by a lower boundary and that there should be a high decrease in intensity close to the perimeter of the reflections. The final segmentation of specular reflections is then given by .

#### 3. Results

In this section, we evaluate the specular reflection algorithms outlined in the previous sections. The evaluation is performed on a dataset of 49 laparoscopic images taken from 27 patients. The images contain 269 true specular reflections. Ground truth segmentation of specular reflection is given by manual segmentation of the specular reflections. The quality of the resulting segmentation is determined using the Jaccard index [18]: where , , and are the true positives, false positives, and false negatives. The Jaccard index is used to measure the overlap of a given segmentation with the ground truth segmentation. The advantage of this error metric is that the amount of true negatives is not considered; in laparoscopic images, the area of specular reflections is usually very small. Therefore, the specificity might be high although the segmented area is several times larger than the true specular reflection. The parameters for the thresholding technique were determined by maximizing the Jaccard index. Figure 3 shows the results of the parameter optimization. One of the main advantages of the hybrid segmentation is that it detects small reflections which would be missed by thresholding techniques. As the Jaccard index computes the overlap of the segmentation with the ground truth, small reflections will give only slight improvements. To demonstrate that many small reflections are detected which would be missed by thresholding, we state the sensitivity in terms of specular reflections. As the number of true negative reflections is unknown—we only have background and specular reflections—the specificity cannot be computed. Therefore, we use the positive prediction value (PPV) to consider the false positives: where and are the number of true and false reflections detected by the segmentation algorithms. Furthermore, we use the the average Jaccard index and the quartiles , of the distribution of the Jaccard index to determine the robustness of the segmentation algorithms. The results are given in Table 1. The results demonstrate the limited accuracy that can be achieved by thresholding techniques for specular reflection segmentation; the cause for this upper limit is an overlap in the color space between specular reflections and the brightest part of the tissue. However, even the adaptive thresholding technique outlined in Section 2.1 just slightly improves accuracy. One reason for that is the assumption that the specular reflections are located in a single peak of the histogram. This assumption is not fulfilled if reflections of Type 1 and Type 3 are present in one image.

The hybrid approach using closed contours and thresholding achieves the highest sensitivity and the best segmentation results in terms of the Jaccard index. The sensitivity is increased by 16% and by 0.17 compared to the best thresholding results. Small weak reflections are detected by their closed contours and give rise to this improvement. Furthermore, the closed contours increase the accuracy of the segmentation for specular reflections of Type 2 which explains the higher Jaccard index. However, there are some closed contours that are falsely classified as specular reflections and cause the PPV to drop slightly compared to the specular peak thresholding and the cone thresholding algorithm.

#### 4. Conclusion

In this section, it was shown that using a hybrid approach combining closed contours and thresholding significantly improves the segmentation of specular reflection in laparoscopic videos. The closed contour computation is performed using the Canny edge detector and the morphological closing operation. This process has the disadvantage that not all the contours of specular reflections will be closed and might be missed. Using approaches that always produce closed contours such as polar transformations and shortest path computations [19, 20] could increase the sensitivity. However, many open contours are not caused by specular reflections; in a brief test, closing open contours using polar transformation increased the false positives in a large scale. Therefore, we apply morphological operations to obtain all contours that contain only small gaps. This leads to a tradeoff between a high sensitivity and an adequate PPV.

#### References

- S. A. Shafer, “UUsing color to separate reflection components,”
*Color Research and Application*, vol. 10, no. 4, pp. 210–218, 1985. View at Google Scholar · View at Scopus - T. Gevers and H. Stokman, “Classifying color edges in video into shadow-geometry, highlight, or material transitions,”
*IEEE Transactions on Multimedia*, vol. 5, no. 2, pp. 237–243, 2003. View at Publisher · View at Google Scholar · View at Scopus - G. J. Klinker, S. A. Shafer, and T. Kanade, “The measurement of highlights in color images,”
*International Journal of Computer Vision*, vol. 2, no. 1, pp. 7–32, 1988. View at Publisher · View at Google Scholar · View at Scopus - J. B. Park and A. C. Kak, “A truncated least squares approach to the detection of specular highlights in color images,” in
*Proceedings of the IEEE International Conference on Robotics and Automation (ICRA '03)*, vol. 1, pp. 1397–1403, Taiwan, September 2003. View at Scopus - A. Artusi, F. Banterle, and D. Chetverikov, “A survey of specularity removal methods,”
*Computer Graphics Forum*, vol. 30, no. 8, pp. 2208–2230, 2011. View at Publisher · View at Google Scholar · View at Scopus - C. L. Novak and S. A. Shafer, “Anatomy of a color histogram,” in
*Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition CVPR '92*, pp. 599–605, IEEE, 1992. - P. Tan, S. Lin, L. Quan, and H.-S. Shum, “Highlight removal by illumination-constrained inpainting,” in
*Proceedings of the 9th IEEE International Conference on Computer Vision*, pp. 164–169, October 2003. View at Scopus - D. Stoyanov and G. Z. Yang, “Removing specular reflection components for robotic assisted laparoscopic surgery,” in
*Proceedings of the IEEE International Conference on Image Processing (ICIP '05)*, pp. 632–635, September 2005. View at Publisher · View at Google Scholar · View at Scopus - O. El Meslouhi, M. Kardouchi, H. Allali, T. Gadi, and Y. Benkaddour, “Automatic detection and inpainting of specular reflections for colposcopic images,”
*Central European Journal of Computer Science*, vol. 1, pp. 341–354, 2011. View at Publisher · View at Google Scholar - F. Vogt, D. Paulus, and H. Niemann, “Highlight substitution in light fields,” in
*Proceedings of the International Conference on Image Processing (ICIP '02)*, vol. 1, pp. 637–640, September 2002. View at Scopus - C.-A. Saint-Pierre, J. Boisvert, G. Grimard, and F. Cheriet, “Detection and correction of specular reflections for automatic surgical tool segmentation in thoracoscopic images,”
*Machine Vision and Applications*, vol. 22, no. 1, pp. 171–180, 2011. View at Publisher · View at Google Scholar · View at Scopus - C. R. Brice and C. L. Fennema, “Scene analysis using regions,”
*Artificial Intelligence*, vol. 1, no. 3-4, pp. 205–226, 1970. View at Google Scholar · View at Scopus - M. Arnold, A. Ghosh, S. Ameling, and G. Lacey, “Automatic segmentation and inpainting of specular highlights for endoscopic imaging,”
*Eurasip Journal on Image and Video Processing*, vol. 2010, article 9, Article ID 814319, 2010. View at Publisher · View at Google Scholar · View at Scopus - J. Oh, S. Hwang, J. Lee, W. Tavanapong, J. Wong, and P. C. de Groen, “Informative frame classification for endoscopy video,”
*Medical Image Analysis*, vol. 11, no. 2, pp. 110–127, 2007. View at Publisher · View at Google Scholar · View at Scopus - T. H. Stehle, “Specular reflection removal in endoscopic images,” in
*Proceedings of the 10th International Student Conference on Electrical Engineering*, Prague, Czech Republic, 2006. - M. Ebner,
*Color Constancy*, vol. 7, Wiley, New York, NY, USA, 2007. - J. Canny, “A computational approach to edge detection,”
*IEEE Transactions on Pattern Analysis and Machine Intelligence*, vol. 8, no. 6, pp. 679–698, 1986. View at Google Scholar · View at Scopus - P. Jaccard, “Etude comparative de la distribution florale dans une portion des alpes et des jura,”
*Bulletin Del La Societe Vaudoise Des Sciences Naturelles*, vol. 37, pp. 547–579, 1901. View at Google Scholar - A. Mishra, Y. Aloimonos, and C. L. Fah, “Active segmentation with fixation,” in
*Proceedings of the 12th International Conference on Computer Vision*, pp. 468–475, IEEE, 2009. - S. J. Chiu, C. A. Toth, C. B. Rickman, J. A. Izatt, and S. Farsiu, “Automatic segmentation of closed-contour features in ophthalmic images using graph theory and dynamic programming,”
*Biomedical Optics Express*, vol. 3, no. 5, pp. 1127–1140, 2012. View at Google Scholar