Comparison and Supervised Learning of Segmentation Methods Dedicated to Specular Microscope Images of Corneal Endothelium

Algorithm 2

Angulo and Matou’s algorithm for detecting the cells [5].

Data: ← Greyscale image of cornea endothelial cells.

← Structuring element sizes.

← Size of Gaussian filter.

← Threshold value.

← Value for the -maxima.

Result: : Segmentation of the cornea endothelial cells.

(1)  begin

(2)  ← Gaussian filter of ;

(3)  ← Opening of with ;

(4)  ← Top-Hat of with ;

(5)  ← Binarization of with threshold ;

(6)  ← Skeletonization of ;

(7) DM ← Distance Map from ;

(8)  ← -maxima of DM (markers);

(9)  ← Watershed constrained by the markers in the inverse of DM;

(10) ← Pruning of to eliminate the lines touching the borders of the image;

(11)  end