Comparison and Supervised Learning of Segmentation Methods Dedicated to Specular Microscope Images of Corneal Endothelium

Algorithm 3

Gavet and Pinoli’s algorithm for detecting the cornea endothelial cells.

Data: ← Greyscale image of corneal endothelial cells.

← Order of alternate sequential filter.

← Length of segments.

← Structuring element size for a closing operation.

← Structuring element size for an opening operation.

Result: Segmentation of the cornea endothelial cells.

(1)  begin

(2) ASF ← Alternate Sequential Filter of size of ;

(3)  ← (−ASF) < 0 (residu computation);

(4)  ← Supremum (union) of Openings of by segments of length ;

(5)  ← Closing of (size );

(6) SK ← Skeletonization of ;

(7) DM ← Distance Map of SK;

(8)  ← Regional Maxima of DM;

(9)  ← Opening of of size ;

(10) ← watershed constrained by markers on inverse of DM;

(11)  ← Pruning of to eliminate the lines touching the borders of the image;

(12) end