Research Article

Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices

Figure 4

MSC morphology and self-renewal. MSC from an 18-year-old healthy donor were cultured on 10 mg/mL fibrin for 7 days before re-seeding them onto different concentrations of fibrin or TCP. We call these secondary colonies. The different surfaces were again tissue culture plastic, 3 mg/mL fibrin gel, 10 mg/mL, and 30 mg/mL (d). MSC from a 55-year-old donor are cultured on TCP and 30 mg/mL fibrin gel. Colony numbers (a) as well as size (b) are shown and the different morphologies (c–h) are shown. *Denotes a statistically significant difference from the other groups.
378034.fig.004a
(a) Sec. young hMSC
378034.fig.004b
(b) Sec. young hMSC
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(c) TCP young hMSC
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(d) Fibrin (10) young hMSC
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(e) Fibrin (3) young hMSC
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(f) Fibrin (30) young hMSC
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(g) TCP old hMSC
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(h) Fibrin (30) old hMSC