Figure 4: Cell viability, proliferation and RNA content of MC3T3-E1 cells (a) LDH activity measured from culture media collected after 2, 7 and 14 days of culture. Data expressed as a relative to the mean value of the TCP group at day 2, which was set as 100%. (b) Number of nuclei were counted with the ImageJ software and referred to the number of cells/mm2. (c) RNA content of cells attached after 2, 7 and 14 days of culture. Values represent the mean ± SEM. Student’s -test: * versus TCP; # versus TiOblast.