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Figure 7: Relative mRNA levels of transcriptional factors and regulators (Hes1, Dlx2, Osx, Smad1, Smad5) after 2, 7 and 14 days of culture. Ratios of target genes relative to housekeeping genes (GAPDH, B-actin) were expressed relative to the mean value of the TCP group at day 2, which was set as 100%. Values represent the mean ± SEM. Differences between groups ( ) were assessed by Student’s -test. # versus TiOblast; * versus TCP.